Composite

Part:BBa_K4343094:Design

Designed by: Chen Hao   Group: iGEM22_NNU-China   (2022-10-09)


hisG-URA-hisG selection marker+ YAT promoter+ EhElo9+ Cyc1t terminator+homologous arm SCP2-up


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 6387
    Illegal NheI site found at 6742
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1431
    Illegal XhoI site found at 3465
    Illegal XhoI site found at 3498
    Illegal XhoI site found at 8556
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3653
    Illegal NgoMIV site found at 7616
    Illegal AgeI site found at 889
    Illegal AgeI site found at 1543
    Illegal AgeI site found at 1642
    Illegal AgeI site found at 2003
    Illegal AgeI site found at 4706
    Illegal AgeI site found at 5067
    Illegal AgeI site found at 6611
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 1546
    Illegal BsaI site found at 2183
    Illegal BsaI site found at 5247
    Illegal BsaI site found at 5784
    Illegal BsaI site found at 6942
    Illegal BsaI site found at 7765
    Illegal BsaI.rc site found at 401
    Illegal BsaI.rc site found at 1540
    Illegal BsaI.rc site found at 6936
    Illegal BsaI.rc site found at 7759
    Illegal BsaI.rc site found at 8029
    Illegal BsaI.rc site found at 9191
    Illegal BsaI.rc site found at 9575
    Illegal SapI.rc site found at 1908
    Illegal SapI.rc site found at 4972


Design Notes

none


Source

from Emiliania huxleyi

References